So here's what SLU used:
- NT2RepCT solution (dope) 100-500 mg/ml
- NaC₂H₃O₂ 100 mM
- Round Glass Capillaries (G1, Narishige) outer: 1mm; inner: .6mm
- Pulled to diameter of 10-30 𝜇m
- 1ml syringe with Luer Lok tip (BD)
- 27 G steel needle(Braun) outer: .4mm
- connect to capillary via polyethylene tubing
- neMESYS low pressure (290 N) syringe pump (Cetoni)
What I plan to actually use/need:
- NT2RepCT solution (dope) 100-500 mg/ml
- NaC₂H₃O₂ 100 mM
- Round Glass Capillaries (G1, Narishige) outer: 1mm; inner: .6mm
- Pulled to diameter of 10-30 𝜇m
I will ask Dr. Rising (nicely) for the NT2RepCT solution. I can easily get the sodium acetate solution from a chemical supplier. The capillaries I would have to buy somewhere... I can't find them cheap enough. I can buy them, but they're $142.00 a pop. So, no. I'd probably break it anyway. (for a comparison: the average human hair is 17-181 𝜇m, that capillary is 10-30 𝜇m!) I'll have to find another solution...
If I can't get the dope from SLU, I may try creating a solution of silk dissolved in acid (...?). Or just build a prototype and talk about it.
All in a day's work
- Noah
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